Anti-Chromatin-Modifying Protein 2a from rabbit

Charged multivesicular body protein 2a (UniProt: O43633; also known as Chromatin-modifying protein 2a, CHMP2a, Putative breast adenocarcinoma marker BC-2, Vacuolar protein sorting-associated protein 2-1, Vps2-1, hVps2-1) is encoded by the CHMP2A (also known as BC2, CHMP2) gene (Gene ID: 27243) in human. CHMP2a is a core component of the endosomal sorting required for transport complex III (ESCRT-III) that is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. It localizes to the mid-body of dividing cells. CHMP2a consists of an acidic C-terminus and the basic N-terminus that render the protein in a closed, soluble and inactive conformation through an auto-inhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components. Cells lacking CHMP2A exhibit mitotic defects. They form transient monopolar spindles and fail to segregate chromosomes normally and also fail to complete abscission. Cell death is also reported to be higher in cells depleted of CHMP2A, CHMP4B, and VPS4B.