Anti-DNMT3L from rabbit

DNA (cytosine-5)-methyltransferase 3-like (UniProt: Q9CWR8; also known as DNMT3L) is encoded by the Dnmt3l gene (Gene ID: 54427) in murine species. DNMT3L is a catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context.It is shown to be essential for the function of DNMT3A and DNMT3B. It activates DNMT3A and DNMT3B by binding to their catalytic domain. It acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. DNMT3L recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. In germ cells, it is required for the methylation of imprinted loci together with DNMT3A. In male germ it is essential for the methylation of retrotransposons, which prevents their mobilization. DNMT3A also plays a key role in embryonic stem cells (ESCs) by acting both as a positive and negative regulator of DNA methylation. It promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B. It can also serve as an inhibitor of DNA methylation at the promoter of bivalent genes. It is reported to promote differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression. In testicular cells it is first observed in non-dividing prospermatogonia after 12.5 dpc and its level reaches its highest at about the time of birth and then rapidly declines and is non-detectable six days postpartum when most of the prospermatognia are differentiated into dividing spermatogonial stem cells.