Anti-PMCA4b Antibody, clone JA3 clone JA3, from mouse

Plasma membrane calcium-transporting ATPase 4 (UniProt: P23634; also known as EC: 3.6.3.8, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4) is encoded by the ATP2B4 (also known as ATP2B2, MXRA1) gene (Gene ID: 493) in human. PMCA4 is a multi-pass membrane protein that acts as a calcium/calmodulin-regulated and magnesium-dependent enzyme and catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCA4 contains 2 calmodulin binding regions known as subdomain A (aa 1086-1103) and B (aa 1104-1113) and a PDZ domain-binding motif that lies within the last 7 amino acids of the carboxy terminus, this latter is absent in the PMCAa tail. PMCA4 is shown to be a regulator of neuronal nitric-oxide synthase (nNOS) and is suggested to play a structural role in tethering nNOS to a highly compartmentalized domain in the cardiac cell membrane. PMCA4b is quite ubiquitousi is a major PMCA expressed in developing mammary tissue. The kinetics of binding of Ca2+/CaM to PMCA4b is very slow, but remains active for longer periods when compared to PMCA4a. A significantly reduced migration of PCMA4b expressing in B-RAF mutant cells is linked to their reduced metastatic potential. (Ref.: Strehler, EE. (2015). Biochem. Biophys. Res. Commun. 460(1); 26-33; Afronze, T., et al. (2014). J. Biol. Chem.289 (10), 7221-31); Mohanmed TM et al. (2011). J. Biol. Chem. 286(48):41520-29).