Anti-RagA/B Antibody, clone 1H7.1 clone 1H7.1, from mouse

Ras-related GTP-binding protein A (UniProt Q7L523; also known as Adenovirus E3 14.7 kDa-interacting protein 1, FIP-1. Rag A, RagA) is encoded by the RRAGA gene (Gene ID 10670) in human. Mammalian cells express four Rag GTPases that mediate amino acid sufficiency-dependent mTORC1 activation to support cell growth and proliferation. Rag GTPases assemble into heterodimers containing GTP-loaded RagA or RagB partnered with GDP-loaded RagC or RagD. Promoted by signal from an upstream amino acid-responsive supercomplex, the active Rag heterodimer accumulates on the cytoplasmic surface of the lysosome, where it recruits mTORC1 and initiates its activation. The pentameric Ragulator complex tethers Rag heterodimers to the lysosomal membrane and acts as RagA/B guanine nucleotide exchange factor (GEF), while the heterotrimeric GATOR1 complex and the heterodimeric FNIP-Folliculin complex function as RagA/B and RagC/D GTPase activating protein (GAP), respectively. Both lysosomal amino acid sensors, such as the multisubunit vacuolar ATPase (v-ATPase) and lysosomal amino acid transporter(s), and cytoplasmic amino acid sensors, such as the leucyl-tRNA synthetase (LeuRS), are part of the upstream amino acid-responsive supercomplex.