Anti-TAF-15 Antibody, clone 12F11 clone 12F11, from rat

TATA-binding protein-associated factor 2N (UniProt: Q92804; also known as 68 kDa TATA-binding protein-associated factor, TAF(II)68, TAFII68, RNA-binding protein 56, TAF15) is encoded by the TAF15 (also known as RBP56, TAF2N) gene (Gene ID: 8148) in human. TAF15 is a ubiquitously distributed protein that is found in all fetal and adult tissues. It is a RNA and ssDNA-binding protein that plays specific roles during transcription initiation at distinct promoters and can enter the preinitiation complex together with the RNA polymerase II (Pol II). TAF15 shuttles between the nucleus and the cytoplasm. It can be dimethylated by protein arginine N-methyltransferase 1 (PRMT1) at Arg206 to asymmetric dimethylarginine. This methylation favors its nuclear localization and it also considered to be essential for its gene regulatory function. TAF15 contains an N-terminal activation domain, an RNA recognition motif (RRM) and many Arg-Gly-Gly (RGG) repeats at its C-terminal end. Its N-terminus serves as an essential transforming domain in the fusion oncoprotein created by chromosomal translocation in certain human chondrosarcomas. Depletion of TAF15 has a growth-inhibitory effect and results in increased apoptosis. (Ref.: Ballarino, M et al. (2013). Oncogene 32(39); 4646-4655; Jobert, L., et al. (2008). Exp. Cell res. 315(7);1273-1286).