Anti-VGluT1 from rabbit

Vesicular glutamate transporter 1 (UniProt: also known as VGluT1, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7) is encoded by the Slc17a7 (also known as Bnpi, Vglut1) gene (Gene ID: 116638) in rat. VGLUT1 is a multi-pass synaptic vesicle membrane protein that mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neuronal cells. Glutamate transporters mediate glutamate uptake into synaptic vesicles and are driven by a proton electrochemical gradient generated by the vacuolar H+-ATPase. Two isoforms of VGLUT1 have been reported that are produced by alternative splicing. VGLUT1 is expressed in the cerebellum, cerebral cortex and hippocampus. Expression of isoform VGLUT2 increases sharply during the first ten days of postnatal life, and also increases with increasing numbers of rhodopsin cells in the retina. It has been shown that both VGLUT1 and VGLUT2 are expressed prominently in the adult brain, however, during the first two weeks of postnatal development, VGLUT2 expression predominates. VGLUT1 expression becomes essential for survival at the time of the developmental switch from VGLUT2 to VGLUT1. The VGLUT1 knockout mice die at the age when a strong upregulation of VGLUT1 through newly emerging VGLUT1 synapses as well as a replacement of the previously predominant VGLUT2 isoform in cerebellar, hippocampal, and cortical neurons occurs. (Ref.: Wojcik, SM et al. (2004). Proc. Natl. Acad. Sci. USA 101(18): 7158-7163).