SIMMONS' CITRATE AGAR ACC. I 500 G

GranuCult(R) prime SIMMONS Citrate Agar is a nutrient rich synthetic medium which is designed for the differentiation of Gram-negative bacteria, primary among the Enterobacteriaceae, from food and animal feed, water and other materials, based on citrate utilization.This culture medium is used for the ability of an organism to utilize citrate as the sole source of carbon and inorganic ammonium salt as sole source of nitrogen for metabolism with resultant alkalinity. The alkaline reaction is evidenced by a change in the colour of the pH indicator bromothymol blue from green (neutral pH) to blue (alkaline pH).Simmons citrate agar is a synthetic modification of Koser's medium with the addition of 1.5% agar and the pH indicator bromothymol blue.Sodium citrate is the sole source of carbon and ammonium dihydrogen phosphate is the sole source of nitrogen. Magnesium sulfate is a cofactor for various metabolic reactions and sodium chloride maintains the osmotic balance. Bromothymol blue is the pH indicator and agar the solidifying agent.