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MISSION(R) Lenti microRNA, Human hsa-miR-6878-5p

ITEM#: 3042-HLMIR2625

MFR#: HLMIR2625

Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr

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Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.
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Product Name Description Item# QTY
MISSION(R) Lenti microRNA, Human hsa-miR-6878-3p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR2624 EA
MISSION(R) Lenti microRNA, Human hsa-miR-520a-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0694 EA
MISSION(R) Lenti microRNA, Human hsa-miR-185-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0283 EA
MISSION(R) Lenti microRNA, Human hsa-miR-196b-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0325 EA
MISSION(R) Lenti microRNA, Human hsa-miR-27a-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0421 EA
MISSION(R) Lenti microRNA, Human hsa-miR-204-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0358 EA
MISSION(R) Lenti microRNA, Human hsa-miR-224-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0403 EA
MISSION(R) Lenti microRNA, Human hsa-miR-1228-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0076 EA
MISSION(R) Lenti microRNA, Human hsa-miR-664b-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-HLMIR0381 EA
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