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MISSION(R) Lenti microRNA, Mouse mmu-miR-6418-3p

ITEM#: 3042-MLMIR1390

MFR#: MLMIR1390

Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr

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Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a proprietary algorithm. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Each miRNA construct has been cloned and sequence verified. Mature microRNA sequences are obtained from miRBase.Lentiviral transduction particles are produced from sequence-verified lentiviral plasmid vectors. Oligos containing the microRNA sequences are cloned into the TRC2-pLKO-puro vector. Co-transfection of this vector into the appropriate cell line with compatible packaging plasmids produces viral particles that can be used to transduce mammalian cells. The polymerase II promoter, elongation factor 1 alpha (EF1A), was chosen to drive miRNA expression needed for reverse transcription of viral RNA and integration of viral DNA into the host cell genome. Additionally, the Woodchuck Hepatitis Post-Transcriptional Regulatory element allowing for enhanced expression of transgenes delivered by lentiviral vectors. This lentiviral vector also carries a puromycin resistance gene for selection of cells. Unlike murine-based MMLV or MSCV retroviral systems, lentiviral-based particles permit efficient infection and integration of the specific miRNA construct into differentiated and non-dividing cells, such as neurons and dendritic cells.
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Product Name Description Item# QTY
MISSION(R) Lenti microRNA Inhibitor, Mouse mmu-miR-6418-3p Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. mi 3042-MLTUD1390 EA
MISSION(R) Lenti microRNA, Mouse mmu-miR-6418-5p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-MLMIR1389 EA
MISSION(R) Lenti microRNA Inhibitor, Mouse mmu-miR-6418-5p Individual lenti microRNA inhibitors are designed using a proprietary algorithm, which is based on the work of Haraguchi, T, et al. and in collaboration with Dr. Hideo Iba, University of Tokyo. This algorithm utilizes the tough decoy (TuD) design. mi 3042-MLTUD1389 EA
MISSION(R) Lenti microRNA, Mouse mmu-miR-6516-3p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-MLMIR1441 EA
MISSION(R) Lenti microRNA, Mouse mmu-miR-344g-3p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-MLMIR1130 EA
MISSION(R) Lenti microRNA, Mouse mmu-miR-489-3p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-MLMIR0487 EA
MISSION(R) Lenti microRNA, Mouse mmu-miR-6912-3p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-MLMIR1481 EA
MISSION(R) Lenti microRNA, Mouse mmu-miR-466i-3p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-MLMIR0837 EA
MISSION(R) Lenti microRNA, Mouse mmu-miR-3095-3p Sigma's Mission Lenti-miRs express miRNAs from a common backbone, whose structure meets requirements for accurate Dicer processing and a partially complementary strand is designed to mimic the base pairing pattern in the backbone structure using a pr 3042-MLMIR1112 EA
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