TagGFP2 Simplicon RNA

Simplicon is a novel system to effect immediate high sustained protein expression of the target gene(s) without the risk of integration. The technology employs a self-replicating RNA based on the Venezuelan equine encephalitis (VEE) genome1. The Simplicon RNA contains only genes encoding the VEE RNA replication machinery while the structural proteins that are required to make an infectious particle have been completely removed and replaced with the transgene(s) of interest. Because Simplicon is a synthetic RNA, there is no risk of the transgene(s) integrating into the transfected cell's genome. Moreover, the self-replicating nature of the Simplicon means that the target protein is continuously expressed in the presence of B18R protein and the selective agent, puromycin. The Simplicon technology has been successfully utilized for efficient human iPSC generation through the sustained expression of critical reprogramming factors, OKSG and OKSG-cMyc1.The Simplicon TagGFP2 RNA Kit was developed to enable the evaluation of the Simplicon expression system in targeted cell(s) before ordering a custom Simplicon RNA. Simplicon TagGFP2 RNA Kit may be used to determine optimal transfection conditions to express the self-replicating RNA in hard-to transfect somatic or primary cells. Simplicon TagGFP2 expresses an improved variant of the Aequorea macrodactyla GFP-like protein. TagGFP2 exhibits bright green fluorescence comparable to that of EGFP, with excitation/emission maxima at 483 and 506 nm, respectively.