Tryptophan (TRP) is one of the eight essential amino acids. In general, TRP is the least abundant amino acid in humans. Chemically, TRP side chain (indole) confers its unique fluorometric properties. Tryptophan is mainly used for protein synthesis, in addition, TRP serves as a building block for several metabolites including kynurenine, serotonin, tryptamine, melatonin, niacin, and NAD/NADP. TRP is the only amino acid that can be found in blood in two forms: bound (BTRP) and free (FTRP) Tryptophan. Changes in tryptophan concentrations are directly related to a number of physiological and behavioral processes including: sleep, memory, depression, motion sickness, bipolar disorders, and schizophrenia. External sources of tryptophan include chicken, tuna, bananas, cheese, and chocolate.