AlphaLISA High Performance Human IL-1β Detection Kit, 5,000 Assay Points

The AlphaLISA High Performance (HP) Human IL-1β Detection Kit is designed for the quantitative determination of human IL-1β in serum, buffered solution, or cell culture medium using a homogeneous (no wash steps, no separation steps) assay. This kit is the new and improved version of part number AL220. Interleukin 1 beta (IL-1β) is a central player of the immune response, involved in inflammation at local and systemic levels. IL-1β is synthesized as a 31 kDa precursor that is cleaved by caspase-1 (ICE) into the active 17 kDa form and eventually released into extracellular space. Its production has been reported in many cell types including brain and monocytic and peripheral blood mononuclear cells. After binding to its receptor, IL-1RI, IL-1β triggers a cascade of kinase signaling pathways that lead to the activation of transcription factors like NFκB and AP-1, eventually activating the expression of genes such as MIP-2 and C-reactive protein. Human