AlphaLISA Surefire Ultra Human and Mouse Phospho-PLC G1 (Ser1248), Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra Phospho-PLC G1 (Ser1248) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-PLC G1 (Ser1248) assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-PLC G1 (Ser1248) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate A431 cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 250 ng/mL EGF for 10 minutes and lysed in 4 mL of 1X SureFire Ultra Lysis buffer.