The AlphaLISA SureFire Ultra Human Phopho-Aurora B (Thr232) assay is a sandwich immunoassay for quantitative detection of phopho-Aurora B (Thr232) in cellular lysates using Alpha Technology. . Aurora B, also known as Aurora Kinase B and serine/threonine-protein kinase 12, is an enzyme coded by the AURKB gene. Aurora B is a mitotic checkpoint kinase that plays a pivotal role in the cell cycle, ensuring correct chromosome segregation and normal progression through mitosis. Overexpressed Aurora B is found in many types of human cancers. The AlphaLISA SureFire Human Phospho-Aurora B (Thr232) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Aurora B (Thr232) in cellular lysates, using Alpha Technology. Formats The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 (mu)L reaction volume. The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 (mu)L reaction volume. The 10,000-