AlphaLISA Surefire Ultra Human Phospho-STAT5 (Tyr694/699), Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra ; Phospho-STAT5 (Tyr694/699) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-STAT5 (Tyr694/699) assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-STAT5 (Tyr694/699) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from A431 cells, cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 2 (mu)g/mL EGF for 10 minutes and lysed in 8 mL of 1X SureFire Ultra Lysis Buffer.