AlphaLISA SureFire Ultra Human Total CDK6 Detection Kit, 100 Assay Points

The AlphaLISA SureFire Ultra Human Total CDK6 assay is a sandwich immunoassay for quantitative detection of total CDK6 in cellular lysates using Alpha Technology. Cyclin-dependent kinase 6 (CDK6) is a member of the subfamily of CDKs that coordinate cell cycle progression in mammalian cells. Mitogenic signals, such as growth factors, trigger cells to enter the G1 phase of the cell cycle by inducing Cyclin D synthesis. Cyclin D then interacts with CDK4 and CDK6 to form active complexes. Both activated kinases are then able to mono-phosphorylate the tumor suppressor RB (protein of retinoblastoma), which still binds to transcription factor E2F, but some genes can be transcribed, such as Cyclin E. CDK6 is overexpressed in many cancers such as lymphoma, leukemia, glioblastoma, bladder, pancreas, and prostate. The AlphaLISA SureFire Human Total CDK6 Detection Kit is a sandwich immunoassay for the quantitative detection of total CDK6 in cellular lysates, using Alpha Technology. F