AlphaLISA Surefire Ultra Human Total FGFR1 Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra Total FGF Receptor 1 assay. This control is already provided in the AlphaLISA SureFire Ultra Total FGF Receptor 1 assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Total FGF Receptor 1 Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate AN3-CA cells were cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 0.5 mM Pervanadate for 10 minutes, and lysed in 5 mL 1X SureFire Ultra Lysis Buffer.