AlphaLISA Surefire Ultra Mouse Total SYK Detection Kit, 500 Assay Points

The AlphaLISA SureFire Ultra Mouse Total SYK assay is a sandwich immunoassay for quantitative detection of total SYK in cellular lysates using Alpha Technology. . The cytoplasmic tyrosine/serine kinase known as SYK (Spleen Tyrosine Kinase) is essential for signal transmission, particularly in immune cells such as B cells and microglia (brain-resident macrophages). SYK interacts with the cytoplasmic domains of CD79 subunits or DAP12 when B cell or TREM2/DAP12 receptor complexes are stimulated, respectively, via binding to their distinct phosphorylated receptor motifs, known as "p-ITAMs." SYK is subsequently activated as a result of Tyr525/526 autophosphorylation of its catalytic domain. Due to SYK's subsequent phosphorylation of downstream substrates like BTK, numerous signaling cascades and physiological reactions-including cell activation, proliferation, and survival-are triggered. SYK is implicated in cancer, central nervous system disorders, and autoimmune diseases. The Al