AlphaLISA Surefire Ultra Total Insulin Receptor ? Detection Kit, Control Lysat

Extra control lysate for the AlphaLISA SureFire Ultra ; Total Insulin Receptor β assay. This control is already provided in the AlphaLISA SureFire Ultra Total Insulin Receptor β assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Total Insulin Receptor β Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. The lysate is prepared from CHO cells transfected with the insulin receptor (CHO-INSR cells), cultured to confluence in T175 flasks in 10% FBS containing medium, then treated with 2(mu)g/mL Insulin and 0.1% BSA for 15 minutes and lysed in 4mL of 1X SureFire Ultra Lysis buffer.