HTRF Human and Mouse Phospho-CHK1 (Ser345) Detection Kit, 500 Assay Points

This HTRF kit enables the cell-based quantitative detection of CHK1 phosphorylation at Ser345, which is activated upon DNA damage. This kit enables optimal investigation of the ATR/CHK1 pathway, such as via its selective inhibitors. This HTRF cell-based assay enables the rapid, quantitative detection of CHK1 phosphorylated at Serine 345, as a readout of the ATR/CHK1 signaling pathway upon a DNA damage response (DDR) In response to DNA damage, for example Single Strand Breaks (SSBs), the ATR-Chk1 pathway and replication checkpoint responses are activated, mediating G2/M checkpoints to arrest cell cycle progression and allow extra time for DNA repair. ATR is recruited to track of ssDNA-RPA through its interacting partner, ATRIP, which leads to Chk1 phosphorylation and activation and in turn induces downstream signaling.