HTRF Human and Mouse Phospho-RET (pan) Detection Kit, 50,000 Assay Points

This HTRF kit enables the cell-based quantitative detection of phosphorylated RET on Tyrosines as a readout of neurotrophic factor activation. The HTRF Phospho-RET (pan) cell-based assay conveniently and accurately detects phosphorylated RET on Tyrosine residues. The assay measures both short and long RET isoforms. The proto-oncogene RET (Rearranged during Transfection), also known as c-Ret, is a receptor tyrosine kinase primarily expressed as two isoforms of 1072 & 1114 amino acids. RET activation by Glial cell line-derived neurotrophic Family Ligands (GFL) leads to its dimerization and autophosphorylation on intracellular Tyrosine residues. RET is required for the development of the nervous system and several other tissues. RET alterations have been detected in numerous human cancers. The development of highly selective and potent RET kinase inhibitors is therefore of great interest to treat RET-altered cancers. Recently, RET has also been linked to neurodegeneration.