IFN-lambda (mouse) LANCE Ultra TR-FRET Detection Kit, 10,000 assay points

The LANCE® Ultra Mouse IFN-gamma Detection Kit is designed for detection and quantitation of mouse interferon gamma in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay..The LANCE® Ultra Mouse IFN-gamma Detection Kit is designed for detection and quantitation of mouse interferon gamma in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 5,000 point kit contains enough reagents to run 5,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). No-wash steps, no separation steps TR-FRET technology Sensitive detection High reproducibility Faster time-to-results Easy automation 96-well, 384-well, and 1536-well formats LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. In the mouse, Interferon-gamma (IFN-gamma) is a homodimeric glycoprotein of 133 amino acids. Interferons (IFNs) were discovered due to their antiviral effects. In humans, there are three families of IFNs: IFN type I (IFN-a, ß, omega, epsilon, and K), IFN type II (one single representative, IFN-gamma), and IFN type III (IFN-lambda1-3). Antigens and mitogens stimulate the production of IFN-gamma in Natural Killer and activated helper T lymphocytes. IFN-gamma shows multiple effects. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens and leukocyte adhesion molecules. It also activates macrophages and enhances the secretion of immunoglobulins by B cells. Response to IFN-gamma is mediated by the heterodimeric IFN-gamma receptor. Importantly, IFNs have proven to be effective in the treatment of several viral infections and cancers.