IL-10 (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Interleukin-10 (IL-10) Detection Kit is designed for detection and quantitation of human IL-10 in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay..Formats: Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay Human Interleukin 10 (IL10) is a homodimer composed of two subunits of 18 kDa each. It is produced by various T cell populations, monocytes, macrophages, and different cell types in the liver when stimulated by endogenous or exogenous factors such as stress, exotoxins, tumor necrosis factor-a, and catecholamines. IL10 inhibits interferon-_ synthesis in Th1 cell clones, monocytes and macrophages. IL10 also inhibits antigen presentation to T cells and IL12 production by monocytes. It also impairs the proliferation and cytokine synthesis of CD4+ T cells, without having a direct inhibitory effect on CD8+ T cells. On the other hand, IL10 has a stimulatory effect on B cells, prevents apoptosis and enhances proliferation and differentiation of plasma cells, and inhibits the release of various chemokines by neutrophils. In general, the main biological function of IL10 is to limit and terminate the inflammatory responses, block proinflammatory cytokine secretion and regulate the differentiation and proliferation of several immune cells. IL10 activity is mediated by the heteromeric IL10 receptor (IL-10R), and signals through the tyrosine kinases Jak1 and Tyk2, and STATs. AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.