TBX (TRYPTONE BILE X-GLUCURONIDE) AGAR

The Reflectoquant Lactic acid test measures total lactic acid (sum of D- and L-lactic acid in beverages, e.g. wine, beer and fruit/vegetable juice after dilution. Furthermore the test can be used for the determination of lactic acid in food after pretreatment. Lactic acid (lactate) is oxidized by nicotinamide adenine dinucleotide (NAD) under the catalytic effect of lactate dehydrogenase to a pyruvate. In the presence of diaphorase, the NADH formed in the process reduces a tetrazolium salt to a blue formazan that is determined reflectometrically on the RQflex.