3-Methyladenine Ready made solution >=98% (HPLC), 7.5mg/ml (50mM) in MES buffer, Autophagy inhibitor

3-Methyladenine (3-MA) is a methylated derivative of adenine that has been used as an In vivo marker for the methylation of DNA. 3-Methyladenine and 7-methylguanine are the two main N-methylpurines formed in DNA upon exposure to methylating agents. 3-MA is removed from damaged DNA via the DNA base excision repair pathway, which begins with the enzyme N-methylpurine DNA glycosylase. The dependence of 3-MA repair on nucleotide excision repair in mammalian cell lines has been studied.3-MA have been widely used as autophagy inhibitor based on its inhibitory effect on class I and class III PI3K activity, which is known to be essential for induction of autophagy. 3-MA blocks class I PI3K persistently, whereas its suppressive effect on class III PI3K is transient, understanding the dual role of 3-MA in autophagy thus suggests that caution should be exercised in the application of 3-MA in autophagy study.Furthermore, 3-MA is also capable of making the reduction of lymphocyte number caused by chronic stress rebound, implying that inhibition of autophagy can rescue chronic stress-induced immunosuppression.Autophagy has been reported to play an important role in the 3, 4-Methylenedioxymethamphetamine MDMA-induced neurotoxicity in the mixed neuronal-glial primary culture and it was found that 3-MA ameliorate MDMA-induced autophagy, SERT loss, HPA axis dysfunction, and depressive behaviors.