A549 EGFRvIII Antigen Panel Cell Lines

This product is a panel of two (2) genetically modified cell lines involving the EGFR gene and the tumor-sepcific EGFRvIII extracellular mutation. The panel consists of a EGFR knockout cell line (EGFR-/-) and an EGFRvIIIhigh expression cell line. The EGFR-/- cell line was generated using CompoZr(R) Zinc Finger Nuclease technology, and the EGFRvIIIhigh expression cell line was generated using MISSION(R) lentivirus. The EGFRvIIIhigh cell line expresses EGFRvIII at high levels relative to the EGFR-/- cell line and wild type A549 cells as measured via fluorescence-activated cell sorting (FACS). The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804.