A549 HLA Panel Cell Lines

The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 86012804.This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in an A549 lung carcinoma parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (beta2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines. CompoZr(R) Zinc Finger Nuclease (ZFN) technology was used to generate the beta2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS). MISSION(R) lentivirus was used to generate the ten (10) monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a beta2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis.