Brefeldin A-inhibited guanine nucleotide-exchange protein 1 (UniProt: Q9Y6D6; also known as BIG1, Brefeldin A-inhibited GEP 1, ADP-ribosylation factor guanine nucleotide-exchange factor 1, p200 ARF guanine nucleotide exchange factor, p200 ARF-GEP1) is encoded by the ARFGEF1 (also known as ARFGEP1, BIG1) gene (Gene ID: 10565) in human. BIG1 is expressed in placenta, lung, heart, brain, kidney and pancreas. BIG1 activates ADP-ribosylation factor (ARF) GTPases and is involved in vesicular trafficking. It promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. BIG1 is required for the maintenance of Golgi structure and this function may be independent of its GEF activity. BIG1 and BIG2 display about 74% homology in their amino acid sequence and about 90% identity in their Sec7 domains. BIG1 is phosphorylated by PKA that reduces its GEF activity. It is localized in the cytoplasm in a multiprotein complex along with BIG2. Its nuclear localization has also been reported and it contains a nuclear localization sequence (aa 711-715). In serum starved cells treated with FK506, BIG1 is essentially completely concentrated in nuclei. BIG1 is shown to be a binding partner of myosin IXb and regulates its Rho-GTPase activating protein activity. Here BIG1 and RhoA compete with each other for the binding to myosin IXb, thus resulting in the inhibition of the GAP activity by BIG1. (Ref.: Lin, S et al. (2013). Arterioscler Thromb Vasc Biol. 33(2): e31-e38).
Platelet glycoprotein 4 (UniProt: Q08857; also known as Glycoprotein IIIb, GPIIIB, PAS IV, PAS-4, Platelet glycoprotein IV, GPIV, CD antigen: CD36) is encoded by the cd36 gene (Gene ID: 12491) in murine species. CD36 is a multifunctional glycoprotein
Nuclear receptor subfamily 1 group I member 2 (UniProt O75469; also known as Orphan nuclear receptor PAR1, Orphan nuclear receptor PXR, Pregnane X nuclear receptor, Steroid and xenobiotic receptor, SXR) is encoded by the NR1I2 (also known as BXR, PAR
Bcl-2-like protein 12 (UniProt Q9HB09; also known as Bcl-2-related proline-rich protein, Bcl2-L-12) is encoded by the BCL2L12 (also known as BPR) gene (Gene ID 83596) in human. Bcl-2 family proteins paly either a pro- or anti-apoptotic role in cell d
Golgi membrane protein 1 (UniProt: Q8NBJ4; also known as Golgi membrane protein GP73, Golgi phosphoprotein 2) is encoded by the GOLM1 (also known as C9orf155, GOLPH2, PSEC0242, UNQ686/PRO1326) gene (Gene ID: 51280) in human. GP73 is a single-pass typ
Lipopolysaccharide-responsive and beige-like anchor protein (UniProt: P50851; also known as Beige-like protein, CDC4-like protein) is encoded by the LRBA (also known as BGL, CDC4L, LBA) gene (Gene ID: 987) in human. LRBA is a single-pass membrane pro
Rab5A is a membrane associated Ras related GTPase that is required for endosome fusion with the plasma membrane. Rab5A also contributes to filopodia extension and other cellular cytoplasmic extensions. Rab5A plays important roles in cellular differen
Retinoschisin (UniProt: O15537; also known as X-linked juvenile retinoschisis protein) is encoded by the RS1 (also known as XLRS1) gene (Gene ID: 6247) in human. Retinoschisin is discoidin domain-containing protein that is localized along the extrace
Rho guanine nucleotide exchange factor 9 (UniProt O43307; also known as Collybistin, PEM-2 homolog, Rac/Cdc42 guanine nucleotide exchange factor 9) is encoded by the ARHGEF9 (also known as ARHDH9, EIEE8, KIAA0424) gene (Gene ID 23229) in human. Guani
Cdc42-interacting protein 4 (UniProt Q15642; also known as hSTP, CIP4, Protein Felic, Salt tolerant protein, Thyroid receptor-interacting protein 10, TR-interacting protein 10, TRIP-10) is encoded by the TRIP10 (also known as CIP4, STOT, STP) gene (G