Anti-Itk Antibody, clone 2F12 clone 2F12, from mouse

Tyrosine-protein kinase ITK/TSK (EC 2.7.10.2; UniProt Q08881; also known as IL-2-inducible T-cell kinase, Interleukin-2-inducible T-cell kinase, Kinase EMT) (T-cell-specific kinase, Tyrosine-protein kinase Lyk) is encoded by the ITK (also known as EMT, LPFS1, LYK) gene (Gene ID 3702) in human. Interleukin-2-inducible T-cell kinase (Itk) is a TEC family non-receptor tyrosine kinase that plays an important roles in signal transduction in T-cells. Upon cell surface receptor stimulation, Itk is recruited to the membrane by the adaptor molecule LAT (linker for activated T cells), where Itk is transphosphorylated at Tyr511 by Lck, Itk Tyr180 then takes place for full activation. Once activated, Itk transduces cellular signaling by activating phospholipase Cgamma1 (PLCgamma1), leading to activation of nuclear factor of activated T-cells (NFAT), nuclear factor kappaB (NFkappaB), and mitogen-activated protein kinase (MAPK) pathways. Itk-/- mice are resistant to developing ovalbumin-induced asthma and exhibit reduced cytokines production by iNKT cells, as well as reduced Th2 effector cytokines and IL-17A productin by T-cells.