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MISSION(R) TRC2 pLKO.5-puro TurboGFP(TM) shRNA Control Plasmid DNA shRNA sequence targeting tGFP

ITEM#: 3042-SHC204

MFR#: SHC204

The MISSION(R) TRC2 Control Vector pLKO-puro TurboGFP(TM) shRNA is a 7518 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP(TM). This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The

The MISSION(R) TRC2 Control Vector pLKO-puro TurboGFP(TM) shRNA is a 7518 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP(TM). This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The TurboGFP(TM) shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION(R) TurboGFP(TM) positive control vector in cell lines expressing TurboGFP(TM). TurboGFP(TM) is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids (SHP001). The TRC2 TurboGFP(TM) shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.