This HTRF kit is designed for robust quantification of ERK modulation, phosphorylated at Thr202/Tyr204..The Advanced Phospho-ERK assay kits are designed for the robust and highly sensitive quantification of ERK phosphorylation on Thr202/Tyr204 as an MAPK pathway readout. The simple mix-and-read protocol eliminates all wash steps for faster analysis and high quality output. The Total-ERK1/2 assay is compatible with the buffers from the Phospho or Advanced phospho-ERK kits, so the same lysate can be used for analysis of the total and the phosphorylated protein populations. This kit is optimal for GPCR and RTK targeted screening..