AlphaLISA Surefire Ultra Human and Mouse Phospho-Rb (Ser780), Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra Phospho-Rb (Ser780) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-Rb (Ser780) assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Phospho-Rb (Ser780) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate Jurkat cells were cultured in T175 flasks to approximately 1 x 106 cells/mL in 10% FBS containing medium, then treated with 1.25 mM Pervanadate for 30 minutes and lysed at 3.2 x 106 cells/mL with 1X SureFire Ultra Lysis Buffer.