AlphaLISA Surefire Ultra Human Phospho-Aurora A (Thr288) Detection Kit, Contro

Extra control lysate for the AlphaLISA SureFire Ultra Phospho-Aurora A (Thr288) assay. This control is already provided in the AlphaLISA SureFire Ultra Phospho-Aurora A (Thr288) assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Phospho-Aurora A (Thr288) Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were grown to confluence in T175 flasks in10% FBS containing medium, then treated with 500 nM Nocodazole and 1 ?M Okadaic Acid for 2 hours at 37°C. Cells were lysed in 4 mL of SureFire Ultra Lysis Buffer.