AlphaLISA Surefire Ultra Human Total Aurora B Detection Kit, Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra Total Aurora B assay. This control is already provided in the AlphaLISA SureFire Ultra Total Aurora B assay kit, but can also be ordered separately. This control lysate is intended for use with the AlphaLISA SureFire Ultra Human Total Aurora B Detection Kit or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, A549 cells were grown to confluence in T175 flasks in 10% FBS containing medium, then treated with 50 nM Calyculin A and 2 ?M PMA for 90 minutes at 37°C. Cells were lysed in 4 mL of SureFire Ultra Lysis Buffer.