AlphaLISA Surefire Ultra Total DNA PKcs Detection Kit, 10,000 Assay Points

The AlphaLISA SureFire Ultra Human Total DNA PKcs assay is a sandwich immunoassay for quantitative detection of total DNA PKcs in cellular lysates using Alpha Technology. DNA-PK, a nuclear DNA-dependent serine/threonine protein kinase, consists of two components, a DNA-dependent protein kinase, catalytic subunit (DNA PKcs) and an autoimmune antigen (KU). DNA-PKcs is inactive and relies on KU to direct it to DNA ends and trigger kinase activity where it is required for the non-homologous end joining pathway of DNA repair which rejoins double-strand breaks. DNA repair genes likely underlie many forms of cancer, and reduced expression of DNA PKcs is associated with cancers such as breast, prostate, and gastric. The AlphaLISA SureFire Human Total DNA PKcs Detection Kit is a sandwich immunoassay for the quantitative detection of total DNA PKcs in cellular lysates, using Alpha Technology. Formats The HV (high volume) kit contains reagents to run 100 wells in 96-well format,