ANGPT2 (human) AlphaLISA Detection Kit, 100 Assay Points

The AlphaLISA® immunoassay kit for human ANGPT2 enables the quantitative determination of human Angiopoietin 2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps)..Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample). Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay Angiopoietin 2 (Ang-2; also ANGPT2) is a glycoprotein which contains 478 amino acids when mature. It is a naturally occurring antagonist for both Angiopoietin 1 and TIE2, and expressed only at the sites of vascular remodeling; similar to Angiopoietin 1. Angiopoietin 2, unlike Angiopoietin 1, promotes cell death and disrupts vascularization. But when it is produced in conjunction with vascular endothelial growth factors, or VEGF, it can promote neo-vascularization. This kit has been designed for the detection of Human Angiopoietin 2 in serum and cell culture supernatants. AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.