HTRF Human and Mouse Phospho-CDK1 Thr14 Detection Kit, 500 Assay Points

This HTRF kit enables the cell-based quantitative detection of phosphorylated CDK1 (Cyclin-Dependent Kinase 1) at Thr14, which is an inhibitory phospho-site essential for maintaining genome integrity and preventing DNA damage during the G2-M phase transition. This HTRF cell-based assay conveniently and accurately detects phosphorylated CDK1 at Thr14. CDK1 (Cyclin-Dependent Kinase 1) is a member of the subfamily of CDKs that coordinate cell cycle progression in mammalian cells (also including CDK1, CDK4, and CDK6). CDK1 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis. CDK1 promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins (Cyclin A, Cyclin B). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of CDK1, can be carried out by Wee1 and Myt1 protein kinases. The cdc25 phosphatase may be responsible for the removal of phosphates at Thr14 and Tyr15