HTRF Human and Mouse Total Cyclin D1 Detection Kit, 500 Assay Points

This lysate is a component of the Total Cyclin D1 kit. It may be used as a positive control for Cyclin D1 protein quantification. The Total Cyclin D1 cell-based assay conveniently and accurately detects the levels of total Cyclin D1 within cells. This kit is compatible with the buffers from the phospho-Cyclin D1 (Thr286) kit, so the same lysate can be used for analyses of both the phosphorylated and the total protein populations. Cyclin D family members (including Cyclin D1, Cyclin D2, and Cyclin D3) are important regulators of cell cycle progression. They are integral mediators of growth factor-dependent G1 to S phase progression by playing the role of allosteric regulators of CDK4/6. Cyclin D1 is upregulated in presence of extracellular mitogenic stimuli, and is then rapidly downregulated by proteasomal degradation following its phosphorylation on Thr286 by the kinase GSK-3?. Cyclin D1 is an oncogene which is overexpressed in many human cancers. Abnormal increased levels of Cyc