IFN-gamma2 (human) AlphaLISA Detection Kit, 500 Assay Points

The AlphaLISA® immunoassay kit for human interferon lambda-2 (IFN-lambda2) enables the quantitative determination of human IFN-lambda2 in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps)..Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample). Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. Interferon-lambda2 (IFN-lambda2), also know an IL28A is a cytokine encoded by the IL28 gene and is closely related to IL28B(IFN-lambda3) and IL29(IFN-lambda1). IFN-lambda2 and IFN-lambda3 are 96% homologous and their functional differences are still unknown. IFN-lambda2 plays a critical role in adaptive immune responses and regulates CD8+ T cell functions during vaccination. Clinically, it is involved in the protection from H1N1 viral infection and it can be used for predicting response to hepatitis C treatment with interferon and ribavirin.