IFN-lambda (ovine) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® immunoassay kit for ovine interferon gamma (IFNgamma) enables the quantitative determination of ovine IFNgamma in serum, buffered solution, and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps)..Formats: Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample). Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. Interferon gamma becomes one of important biomarkers for determining infectious diseases in human and in domestic animals. It is critical for innate and adaptive immunity against viral, bacterial and protozoan infections. In domestic animal species, IFNgamma is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Ovine IFNgamma has been identified as a biomarker for vaccine responses, viral, and bacterial infection. This kit is designed to detect oIFNgamma in serum, plasma, and cell culture supernatants.