Phospho-CDK2 (Tyr15) Cellular Kit, 10000 Tests

This HTRF kit enables the cell-based quantitative detection of phosphorylated CDK2 (Cyclin-Dependent Kinase 2) at Tyr15, which is an inhibitory phospho-site essential for maintaining genome integrity and preventing DNA damage during the S phase.This HTRF cell-based assay conveniently and accurately detects phosphorylated CDK2 at Tyr15.  CDK2 (Cyclin-Dependent Kinase 2) is a member of the subfamily of CDKs that coordinate cell cycle progression in mammalian cells (also including CDK1, CDK4, and CDK6). CDK2 is activated by interaction with Cyclin E and Cyclin A during the late G1 phase and S phase respectively, and is also regulated by two major phospho-sites. Phosphorylation at Tyr15 by the kinase Wee1 is inhibitory by preventing ATP binding, whereas Tyr15 dephosphorylation by the phosphatase Cdc25A and phosphorylation at Thr160 by CAK (CDK Activating Kinase) is required for its full activity. CDK2 inhibitory phosphorylation at Tyr15 is essential for maintaining genome integrity and preventing DNA damage during the S phase. The Wee1/Cdc25A axis is therefore an attractive target for cancer therapy and may represent a unique approach to sensitize cancer cells with hyperactive CDK2.