AlphaLISA SureFire Ultra phospho-MKK4 (Ser257) Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra phospho-MKK4 assay. This control is already provided in the AlphaLISA SureFire Ultra phospho-MKK4 assay kit, but can also be ordered separately..This control lysate is intended for use with the AlphaLISA SureFire Ultra phospho-MKK4 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, HEK293 cells were cultured to confluence in T175 flasks in 10% FBS-containing medium, then treated with 1 M Sorbitol for 45 min and lysed in 4 mL of 1X SureFire Ultra Lysis buffer..