AlphaLISA SureFire Ultra Total SMAD2 Control Lysate

Extra control lysate for the AlphaLISA SureFire Ultra total SMAD2 assay. This control is already provided in the AlphaLISA SureFire Ultra total SMAD2 assay kit, but can also be ordered separately..This control lysate is intended for use with the AlphaLISA SureFire Ultra total SMAD2 assay kit, or for use as a control for those running orthogonal or secondary assays along with the SureFire assay. To prepare the lysate, RAW 264.7 cells were cultured to confluence in T175 flasks in 10% FBS-containing medium, then treated with 10 ng/mL rhTGFB for 90min and lysed in 6 mL of 1X SureFire Ultra Lysis buffer..