The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing. CET 1019 HS-puro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element. CET 1019 AS-puro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element. The CET 1019 HS-puro-SceI and CET 1019 AS-puro-SceI vectors possess the following features:• A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzymes to permit easy insertion of any gene of interest (cDNA or genomic)• A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation• A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells• A β-lactamase gene for plasmid selection and propagation in the transformed prokaryotic cells in the presence of ampicillin• An I-Scel homing restriction endonuclease site for linearization of constructs prior to the transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettesTHIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.