Anti-Glycoprotein 78 Antibody, clone 3F3A clone 3F3A, from rat

E3 ubiquitin-protein ligase AMFR (UniProt Q9UKV5; also known as AMF receptor, Autocrine motility factor receptor, gp78, RING finger protein 45) is encoded by the RNF45 (also known as AMFR) gene (Gene ID 267) in human. Glycoprotein 78 (gp78) is an endoplasmic reticulum (ER) membrane-anchored E3 ubiquitin ligase that plays a key role in ER-associated degradation (ERAD). Gp78 is localized to a mitochondria-associated ER domain, where it induces mitochondrial fragmentation by targeting the mitochondrial fusion proteins mitofusin 1 and 2 (Mfn1 and Mfn2, respectively) for degradation upon mitochondrial depolarization. Other known ERAD substrates processed by gp78 include ApoB lipoprotein, HMG CoA reductase, CD3-delta, cystic fibrosis transmembrane conductance regulator, the metastasis suppressor KAI1, unglycosylated prion protein (PrP), and the T-cell receptor. Gp78 is a 7-transmembrane protein (a.a. 82-102, 122-142, 145-165, 186-206, 215-235, 276-296, 429-449) with a RING-type Zinc finger (a.a. 341-379), a CUE domain (a.a. 456-498), and a VCP/p97-interacting motif (VIM; a.a. 622-640).