Anti-Histone H1 Antibody, clone 1G6 clone 1G6, from mouse

Histone H1 (UniProt: P02255; also known as H1) is encoded by the His 1 gene (Gene ID: 318854) in Drosophila species. Histone H1 is a linker histone that is involved in the organization and maintenance of chromatin higher order structure and plays an active role in the control of DNA replication and cell proliferation. The linker histone H1 family members are a key component of chromatin and bind to the nucleosomal core particle around the DNA entry and exit sites. Histone H1 is essential for the condensation of nucleosome chains into higher-order structures. In metazoans, H1 histones are smaller in size with about 200 amino acids. They have a short N-terminal tail, a central globular domain, and a long basic C-terminal tail. The central globular domain is shown to be highly conserved among all H1 subtypes. Manipulating levels of H1 histone can lead to both up- and down-regulation of specific genes. Depletion of H1 results in a massively altered chromosome structure with loss of chromosome banding. In Drosophila, a 20% reduction in H1 content leads to cessation of growth beyond the larval stage. Histone H1 undergoes phosphorylation mainly in the tail region, especially the C-terminal tail, where several {(S/T)-P-X-(K/R)} motifs are located that are recognized by cyclin-dependent kinases (CDKs). Phosphorylation levels are reported to be lowest during the G1 phase of the cell cycle and increase during S phase and reach maximum levels in mitosis. (Ref.: Hergeth, SP., and Schneider, R (2015). EMBO Rep. 16(11); 1439-1453).