Anti-Neogenin Antibody, clone 21A8 clone 21A8, from rat

Neogenin (UniProt: P97798) is encoded by the Neo1 (also known as Ngn) gene in murine species. Neogenin is a single-pass type I membrane protein that is expressed ubiquitously throughout the mid to late stages of gestation and in adult tissues. It strong expression is observed in the ventral region of the ventricular zone of the E15.5 mouse neural tube, as well as in the ventricular zones of the mesencephalon and rhombencephalon. Five different isoforms of neogenin have been described that are produced by alternative splicing. Isoform 3 and isoform 4 are shown to be expressed at higher level compared to other isoforms between E11.5 and E16.5. Neogenin is a multi-functional cell surface receptor that regulates cell adhesion in many diverse developmental processes, including neural tube and mammary gland formation, myogenesis and angiogenesis. It also serves as a receptor for members of the BMP, netrin, and repulsive guidance molecule (RGM) families. Interactions between netrin and neogenin result in a chemoattractive axon guidance response and cell-cell adhesion whereas its interaction with RGMa and RGMb can induce a chemorepulsive response. Neogenin is synthesized with a signal peptide (aa 1-36), which is subsequently cleaved off to produce the mature form that has an extracellular domain (aa 37-1136), a short transmembrane domain (aa 1137-1157), and a cytoplasmic domain (aa 1158-1493).