Anti-Thymidylate Kinase/TMPK Antibody, clone 1A5.1 clone 1A5.1, from mouse

Thymidylate kinase (EC 2.7.4.9; UniProt P23919; also known as dTMP kinase) is encoded by the DTYMK (also known as CDC8, PP3731, TMPK, TYMK) gene (Gene ID 1841) in human. Thymidylate kinase (TMPK) is an ubiquitously expressed enzyme of the dTTP synthesis pathway involved in DNA synthesis. TMPK catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) in the presence of ATP and Mg(2+) to form thymidine 5'-diphosphate (dTDP), which is then further phosphorylated by nucleoside-diphosphate kinase (NDK) to thymidine 5'-triphosphate (dTTP). Aside from its normal physiological role, TMPK activity is essential for the activation of nucleoside analogs-based prodrugs, such as the clinically used anti-HIV prodrugs AZT and d4T, which need to be converted to the triphosphate forms for their pharmacological activities. Inhibition of TMPK activity is reported to augment the potency of anticancer agent doxorubicin against human colon cancer cells regardless of their p53 status. In addition, lung cancers with LKB1-mutations are reported to show deficits in nucleotide metabolism and exhibit hypersensitivity to DTYMK inhibition.