PSA (human) AlphaLISA Detection Kit, 5,000 Assay Points

The AlphaLISA® Human Prostate-Specific Antigen (PSA) Detection Kit is designed for detection and quantitation of human PSA in serum, buffered solution or cell culture medium in a homogeneous (no-wash steps, no separation steps) assay. PSA is also known as Kallikrein-related peptidase 3 (KLK3)..Formats: Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Features: No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay Human prostate-specific antigen (hPSA, Kallikrein-related peptidase 3, KLK3) is a 33 kDa glycoprotein of the kallikrein family present in seminal fluids. It is a protease that seems to function in the liquefaction of semen after ejaculation, facilitating sperm migration through cervical mucus. Small amounts are normally found in the serum of healthy men, but a number of pathological conditions could increase them due to prostatic tissue damage, hence its quantitation may be helpful in the diagnosis and monitoring of prostatic carcinomas. AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.