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A375 NY-ESO-1 Antigen Panel Cell Lines

ITEM#: 3042-ATG0041KT

MFR#: ATG004-1KT

This product is a panel of three (3) genetically modified cell lines involving the NY-ESO-1 gene. The panel consists of a NY-ESO-1 knockout cell line (NY-ESO-1-/-) as well as a NY-ESO-1low and a NY-ESO-1high expression cell line. The NY-ESO-1-/- cell

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This product is a panel of three (3) genetically modified cell lines involving the NY-ESO-1 gene. The panel consists of a NY-ESO-1 knockout cell line (NY-ESO-1-/-) as well as a NY-ESO-1low and a NY-ESO-1high expression cell line. The NY-ESO-1-/- cell line was generated using CompoZr(R) Zinc Finger Nuclease technology, and the two NY-ESO-1 expression cell lines were generated using MISSION(R) lentivirus. The NY-ESO-1low and NY-ESO-1high cell lines express NY-ESO-1 at low and high levels relative to the NY-ESO-1-/- cell line (NY-ESO-1high > NY-ESO-1low > NY-ESO-1-/-) as measured via fluorescence-activated cell sorting (FACS). As a comparison, wild type A375 cells demonstrate NY-ESO-1medium expression as measured via FACS. The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88113005.
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